témavezető: Dinnyés András
helyszín (magyar oldal): "BioTalentum Kft. 2100 Gödöllő, Szent-Györgyi Albert utca 4. alatt található kutató laboratóriuma" helyszín rövidítés: Bio
A kutatási téma leírása:
"Objectives: Genetic Reprogramming holds great potential for new medical applications, such as cell replacement therapies. Since iPS cells can be made from a patient’s own cells e.g. skin, they could be used to grow specialized cells that exactly match the patient and would not be rejected by the immune system. In theory, if the patient has a genetic disease, which could be corrected in their iPS cells in the laboratory, then these repaired iPS cells used to produce a patient-specific batch of healthy specialized cells for transplantation. Also these cells are suitable for disease modelling or to drug screening. However, currently little is known about the value of reprogramed cells in disease modelling, because only a few disease model were published so far where iPSCs were successfully applied. The lack of knowledge makes it impossible to evaluate the real value of reprogrammed cells both for human or animal disease modelling.
The scope of the current PhD studentship would be the investigation of the potential of reprogrammed cell in the modelling of genetically inheritable type of diseases exists both in animals and humans.
Expected results:
Improved genetic reprogramming of fibroblast or blood cells toward induced pluripotent stem (iPS) cells
Detailed pathomechanism studies of genetically inheritable disorders by iPS differentiation.
Results on the effect of genetic reprogramming on
- cell viability
- terminal differentiation and
- cell functionality of the reprogrammed cells.
Tasks of the PhD student / experimental locations:
The experiments are mostly planned to be performed at the Research Laboratory of BioTalentum Ltd at Gödöllő.
Tasks and methodology:
Culture of mouse and human fibroblast cells.
Reprogramming of fibroblast or blood cells into induced pluripotent stem cells
Characterisation of the established cells to analyse pathomechanism (fluorescent staining for markers, FACS analyses, qRT PCR comparison, functionality studies).
Generation of reporter constructs
Gene targeting in stem cell cultures
From the results generated the successful candidate can publish at least in 2 peer-reviewed journals and at least 2 congress abstracts. At the end of the fellowship period the participant needs to prepare a PhD thesis in line with the rules of the PhD School."
előírt nyelvtudás: English ajánlott nyelvtudás (magyar oldal): Deutsch felvehető hallgatók száma: 1
Jelentkezési határidő: 2015-05-29
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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