The project aims at gathering functionally relevant structural data in yeast vacuolar membranes on the ATP dependent proton pumping of V-ATPase using spectroscopic techniques. The dependence of both the ATP hydrolysis and proton pumping will be studied, primarily in native membranes, as a function of the physical state of the membrane, external conditions (temperature, oscillating electric field) and non-covalent V-ATPase inhibitors. These experiments will provide data for a better understanding of the functioning and regulation of the membrane-bound proton pumping molecular machinery, the V-ATPase.
Required language skills: English Number of students who can be accepted: 1
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