Thesis topic proposal
Gábor Szabó
Accessibility of chromatin through biophysical spectacles


Institute: University of Debrecen
theoretical medicine
Doctoral School of Molecular Cellular and Immune Biology

Thesis supervisor: Gábor Szabó
Location of studies (in Hungarian): Debreceni Egyetem, Általános Orvostudományi Kar
Abbreviation of location of studies: DEÁOK

Description of the research topic:

The euchromatin-heterochromatin dichotomy is a well-known light- and electronmicroscopic correlate of the activity and inactivity of the genome, respectively, generally interpreted in terms of packaging differences that, based on common sense, are expected to reflect or determine chromatin accessibility to regulatory macromolecules. A differential accessibility of the two types of chromatin, however, has never been experimentaly verified. Likewise, no clear correlation with gene activity has been clearly documented for the different levels of chromatin’s structural hierarchy. The only structural feature with unequivocally repressive character is the presence of the nucleosomes on inactive promoters and enhancers opposed to the nucleosome free regulatory regions of the active genes, and the relatively decreased stability of nucleosomes along the transcribed loci. Using a laser scanning cytometric approach recently elaborated in our lab for the analysis of nucleosome eviction in a histone modification and cell-cycle dependent manner in situ, combined with fluorescence resonance energy transfer methodology we set out to fill the above, crucial conceptual gap in our understanding of the main principles of gene regulation in higher eukaryotes involving the human.

Deadline for application: 2017-01-15

2017. I. 31.
ODT ülés
Az ODT következő ülésére 2017. március 10-én 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).

All rights reserved © 2007, Hungarian Doctoral Council. Doctoral Council registration number at commissioner for data protection: 02003/0001. Program version: 1.2334 ( 2017. I. 15. )